Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017630 | GenBank Accession | JX982976 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 312 |
| Definition | Human bocavirus isolate Taif-1 NP1 protein (NP1) gene, partial cds. | Submission Date | 2012-10-18 |
| Strain Name | Taif-1 | Sampling Date | 2012-01-10 |
| Sampling Country | Saudi Arabia | Location | he Governmental Pediatric Hospital- Al-Taif, Saudi Arabia, Saudi Arabia |
| Submitted By | Abdel-Moneim,A.S., Kamel,M.M., Al-Ghamdi,A.S. and Al-Malky,M.I.R. | Submitting Institution | Microbiology Department, Virology Division, College of Medicine, Taif University, Al-Taif, Saudi-Arabia |
| Original Host | Homo sapiens | Sample Type | Nasopharyngeal swabs |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Gentamycin sulphate | Preservative in sterile saline for nasopharyngeal swabs. | 23383205 | View Related Publication |
| 2 | DNA/RNA extraction Kit (Koma Bioteck Inc., Seoul, Korea) | Extraction of viral nucleic acid from individual samples. | ||
| 3 | Real-time PCR bocavirus detection Kit (Liferiver, Shanghai, China) | Detection and amplification of human bocavirus DNA during RT PCR. | ||
| 4 | Enzyme mix | Used in the real-time PCR reaction for DNA amplification. | ||
| 5 | Real-time PCR Kits (Shanghai ZJ Bio-tech Co., Ltd) | Screening for respiratory syncytial virus (RSV), influenza A virus (IAV), parainfluenza virus 1 (PIV-1), and parainfluenza virus 3 (PIV-3), as well as respiratory enteric virus (AdenV). | ||
| 6 | DNA master mix (Koma Bioteck, Inc., Seoul, Korea) | Used in PCR amplification of NP1 gene for HBoV genotyping. | ||
| 7 | QIAquick PCR purification kit (Qiagen) | Purification of positive PCR products before sequencing. |