ParvoDB ID PV017624 GenBank Accession KU360110
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 410
Definition Human bocavirus 1 isolate EG-BSU/CRC-22 coat protein VP1 gene, partial cds. Submission Date 2015-12-27
Strain Name EG-BSU/CRC-22 Sampling Date 2012-11-23
Sampling Country Egypt Location Egypt
Submitted By Abdel-Moneim,A.S., El-Fol,H.A., Kamel,M.M., Soliman,A.S.A., Mahdi,E.A., El-Gammal,A.S. and Mahran,T.Z.M. Submitting Institution Microbiology, Virology Division, College of Medicine, Taif University, Al-Haweyia, Al-Taif 21944, Saudi Arabia
Original Host Homo sapiens Sample Type cancer tissue specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Hematoxylin Staining pathological sections of cancer specimens. 27155943 View Related Publication
2 Eosin Counterstaining pathological sections of cancer specimens.
3 gSYNCTM DNA Extraction Kit (Geneaid Biotech Ltd., Taiwan) DNA extraction from tissue sections.
4 Xylene Deparaffinization of tissue sections.
5 100% Ethanol Deparaffinization of tissue sections and evaporation process.
6 0.1% SDS Component of the DNA extraction buffer.
7 10 mM EDTA Component of the DNA extraction buffer.
8 Proteinase K (62.5 mg/ml) Component of the DNA extraction buffer for digesting proteins.
9 PCR Mastermix (Solis Bio-Dyne, Inc., Estonia) Polymerase chain reaction (PCR) amplification.
10 Gel Extraction Kit (Koma Bioteck, Inc., Seoul, Korea) Purification of positive PCR amplicons.