Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017402 | GenBank Accession | DQ267766 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 240 |
| Definition | Human bocavirus isolate CAN1179-03 nonstructural protein 1 (NP-1) gene, partial cds. | Submission Date | 2005-10-26 |
| Strain Name | CAN1179-03 | Sampling Date | from 2002-01 to 2004-12 |
| Sampling Country | Canada | Location | Canada |
| Submitted By | Bastien,N., Brandt,K., Dust,K., Ward,D. and Li,Y. | Submitting Institution | National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada |
| Original Host | Homo sapiens | Sample Type | throat swabs, nasopharyngeal swabs, nasopharyngeal aspirates, and auger suctions |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | BioRobot MDx | Used for viral DNA extraction from original samples. | 16704852 | View Related Publication |
| 2 | QiAamp Virus BioRobot MDX kit | Used for viral DNA extraction from original samples. | ||
| 3 | HotStartTaq DNA polymerase (Qiagen) | Used to activate the enzyme for the polymerase chain reaction (PCR) thermocycler conditions. | ||
| 4 | ABI 377 Sequencer and fluorescent dye terminator kit (Applied Biosystems) | Used to determine nucleotide sequences of the NP-1 gene amplicons. | ||
| 5 | Primers: 188F and 542R | Used for the initial screening based on the NP-1 gene sequence for HBoV detection by PCR. | ||
| 6 | Primers: VP1/VP2F and VP1/VP2R | Used for confirmation of HBoV detection by PCR based on the VP1/VP2 gene sequences. | ||
| 7 | 12 negative controls per 96-well plate | Used for DNA extraction and PCR procedures to avoid cross-contamination. |