1
Stool collection tubes
Collection and transfer of stool samples for lab analysis.
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2
Phosphate-buffered saline with glycerol (15%)
Dilution buffer for stool samples.
3
AmpliSensRIBO-sorb extraction kit
Total viral nucleic acid extraction.
4
AmpliSensReverta-L reverse-transcription kit
Synthesis of complementary DNA.
5
DreamTaq Green PCR Master Mix (Thermo Scientific, Lithuania)
PCR assays.
6
Mon244/245 primers
Detection of HAstV by amplifying a 413-bp fragment of the ORF2.
7
Agarose gel with ethidium bromide
Electrophoresis separation and visualization of PCR products.
8
Gel Doc XR+Imager System (Bio-Rad Laboratories, Inc.)
Examination of electrophoresis results.
9
Beg9/End9 primers
Amplification and sequencing of the VP7 gene of RVA.
10
4Con3 and 4Con2 primers
Sequencing of the VP4 gene of RVA.
11
VP6F/VP6R and End6 primers
Sequencing of partial VP6 gene of RVA.
12
JV12/JV13, CVR
Sequencing of NoV including the ORF1/ORF2 overlapping region.
13
Mon381F/383R primers
Sequencing of NoV capsid genotype.
14
BigDyeTerminator v.3.1 Cycle Sequencing Kit
Determination of nucleotide sequences.
15
ABI 3500 Genetic Analyzer (Applied Biosystems, CA, USA)
Sequencing analysis.
16
SeaKem GTG-agarose gel (Lonza, ME, USA)
Purification of PCR products.
1
Phosphate buffer
Diluting stool samples.
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2
Ribo-Sorb extraction kit (Interlabservis)
Isolating viral DNA from feces.
3
DreamTaq Buffer (Thermo Scientific)
PCR reaction buffer.
4
dNTP mixture
Provides nucleotides for DNA synthesis during PCR.
5
Primers sf2 and sr2
PCR amplification for HBoV detection.
6
DreamTaq DNA Polymerase (Thermo Scientific)
Enzyme for DNA amplification in PCR.
7
Ribo-Sorb (Interlabservis)
Isolating viral DNA from feces.
8
GeneAmp 9700 amplifier (Applied Biosystems)
PCR machine used for nucleic acid amplification.
9
Agarose gel
Electrophoresis for analyzing PCR products.
10
DNA Gel Extraction kit (Fermentas)
Purifying PCR products for sequencing.
11
BigDye Terminator v. 3.1 Cycle Sequencing kit (Applied Biosystems)
Sequencing the amplified DNA fragments.
12
CentriSep columns (Applied Biosystems)
Purifying sequencing reaction products.
13
3500 Genetic Analyzer DNA sequencer (Applied Biosystems)
Analyzing DNA sequence.
14
Phusion High-Fidelity DNA Polymerase (Thermo Scientific)
High-fidelity enzyme for PCR amplification.
15
Klenow Fragment
Adding A-overhangs to PCR products for cloning.
16
pGEM-T vector (Promega)
Cloning PCR products.
17
T4 DNA ligase
Ligating PCR products into the cloning vector.
18
E. coli XL2-Blue cells
Transformation host for cloning.
19
X-gal
Screening cloned colonies for inserts.
20
IPTG
Inducing β-galactosidase activity for blue/white screening.
21
Ampicillin
Selective antibiotic for bacterial cultures containing the cloning vector.
22
Plasmid Mini Kit (Qiagen)
Isolating recombinant plasmids.
