Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017205 | GenBank Accession | KU362763 |
| Subfamily | Parvovirinae | Genus | Protoparvovirus |
| Species | Protoparvovirus primate1 | Sequence Length | 1710 |
| Definition | Human Bufavirus isolate H232 VP2 protein gene, complete cds. | Submission Date | 2015-12-30 |
| Strain Name | H232 | Sampling Date | 2013 |
| Sampling Country | Tunisia | Location | Monastir, Tunisia, Tunisia |
| Submitted By | Ayouni,S., Belliot,G. and De Rougemont,A. | Submitting Institution | Centre National de Reference des Virus Enteriques, Centre Hospitalier Universitaire de Dijon, 2, rue Angelique Ducoudray, Dijon Cedex 21070, France |
| Original Host | Homo sapiens | Sample Type | stool samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | PBS | Used for making 10% fecal suspension for nucleic acid extraction. | 27631733 | View Related Publication |
| 2 | NucliSens EasyMAG system (bioMérieux, Marcy l’Etoile, France) | Used for the extraction of nucleic acids from stool samples. | ||
| 3 | Qiagen OneStep RT-PCR kit (Qiagen GmbH, Hilden, Germany) | Used for performing reverse transcription-polymerase chain reactions (RT-PCR). | ||
| 4 | Novagen KOD Hot Start polymerase kit (EMD Millipore, Darmstadt, Germany) | Used for performing polymerase chain reactions (PCR). | ||
| 5 | Eppendorf MasterCycler PCR machine (Eppendorf AG, Hamburg, Germany) | Used for amplifying DNA/RNA samples via PCR. | ||
| 6 | ABI Big Dye sequencing kit | Used for sequencing PCR products. | ||
| 7 | ABI 3130XL sequencer (Applied Biosystems, Waltham, USA) | Used for obtaining sequences of PCR products. |