Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017141 | GenBank Accession | JQ618268 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 636 |
| Definition | Human bocavirus isolate Cam2009-E569 capsid protein gene, partial cds. | Submission Date | 2012-02-03 |
| Strain Name | Cam2009-E569 | Sampling Date | from 2007 to 2009 |
| Sampling Country | Cambodia | Location | Takeo (southern Cambodia) and Kampong Cham (central-north Cambodia) provincial hospitals, Cambodia |
| Submitted By | Arnott,A., Vong,S., Rith,S., Naughtin,M., Ly,S., Guillard,B., Deubel,V. and Buchy,P. | Submitting Institution | Virology Unit, Institut Pasteur in Cambodia, 5 Monivong Blvd, PO Box 983, Phnom Penh, Cambodia |
| Original Host | Homo sapiens | Sample Type | nasopharyngeal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp DNA Mini Kit (Qiagen, Valencia, CA, USA) | Extract viral DNA from nasopharyngeal samples | 22531100 | View Related Publication |
| 2 | Qiagen AE buffer | Elute DNA | ||
| 3 | Go-Taq Flexi DNA polymerase (Promega, Madisson, WI, USA) | Perform the thermocycling reactions | ||
| 4 | Ethidium bromide | Visualize PCR products under UV light on a 1.5% agarose gel | ||
| 5 | QIAquick PCR Purification kit (Qiagen) | Purify PCR products prior to sequencing, using the QIAquick Gel Extraction protocol | ||
| 6 | ABI BigDye Terminator Cycle Sequencing kit | Conduct single-pass sequencing reactions |