Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV017105 GenBank Accession DQ471805
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 354
Definition Human bocavirus isolate CHSD4 NP-1 gene, partial cds. Submission Date 2006-03-27
Strain Name CHSD4 Sampling Date 2004-12-27
Sampling Country United States of America Location the Children's Hospital of San Diego, California, United States of America
Submitted By Arnold,J.C., Singh,K.K., Spector,S.A. and Sawyer,M.H. Submitting Institution Ped Inf Disease, UC San Diego, 9500 Gilman Drive, La Jolla, Ca 92093-0672, USA
Original Host Homo sapiens Sample Type nasal scraping specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Saline Used as a medium to place nasal scraping specimens for diagnostic testing. 16804840 View Related Publication
2 QIAamp MinElute Virus Spin Kit (Qiagen) Used for nucleic acid extraction from 50-200 mL of sample.
3 FastStart SYBR Green Master kit (Roche) Used for PCR optimization and screening.
4 Plasmid containing a 560-bp region of the NP-1 region of HBoV Used as a positive control for optimizing the PCR reaction.
5 Primer set targeting the NP-1 region Used for the initial PCR screening for HBoV.
6 Lightcycler instrument (Roche) Used to run the PCR cycles and measure fluorescence.
7 Primer set targeting the NS1 gene Used for the confirmation PCR analysis.
8 3% agarose gel with ethidium bromide Used to locate DNA bands for visualization.
9 QIAquick Gel Extraction Kit (Qiagen) Used for extracting DNA from the agarose gel.
10 Spectrophotometer Used to quantify DNA concentration by measuring absorbance at 260 nm and 280 nm.