Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017041 | GenBank Accession | MK910896 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 205 |
| Definition | Human bocavirus 1 isolate 12-0075 VP1 gene, partial cds. | Submission Date | 2019-05-10 |
| Strain Name | 12-0075 | Sampling Date | 2011 |
| Sampling Country | Mexico | Location | Mexico |
| Submitted By | Arellano-Galindo,J., Arroyo-Gamino,A.E., Contreras-Alvarado,L.M., Hernandez-Castillo,L., Salgado-Servin,I., Trujillo-Ayala,A. and Moreno-Espinosa,S. | Submitting Institution | Virology Laboratory, Hospital Infantil de Mexico Federico Gomez, Dr Marquez 162, Mexico City 06720, Mexico |
| Original Host | Homo sapiens | Sample Type | nasopharyngeal swab |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp DNA minikit (Qiagen code 51306) | DNA extraction following manufacturer’s recommendations. | 38187006 | View Related Publication |
| 2 | RespiFinder 19 | Identification of respiratory pathogens through multiplex RT-PCR test (April 2010 to May 2012). | ||
| 3 | RespiFinder 22 | Identification of respiratory pathogens, including bocavirus (June 2012 to March 2014). | ||
| 4 | Virus-specific primers for HBoV1 | Subsequent testing for HBoV1 on samples initially tested with RespiFinder 19. | ||
| 5 | Inhouse double nest PCR | Confirmation of HBoV1 using virus-specific primers and previously reported conditions. | ||
| 6 | HBoV Real Time PCR Kit (MyBiosource, San Diego Cal.) catalogue MBS598173 | Viral load quantitation of HBoV1 positive samples. | ||
| 7 | Primers previously reported for NP1 and VP/NC analyses | Phylogenetic distribution analysis based on regions of NP-1 and VP/NC. | ||
| 8 | High pure PCR purification kit from Sigma-Aldrich (Cat. 11732668001) | Purification of PCR products. | ||
| 9 | Campbell Scientific environmental sensors | Measurement of environmental temperature and humidity for seasonality analysis. |