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1
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Ultrasense Viral RNA Kit (Qiagen, Germany)
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Isolation of total RNA
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2
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Ribominus Concentration Module (Life technologies, USA)
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Concentration of isolated RNA
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3
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Ribominus Eukaryotic Kit v2 (Life technologies, USA)
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Depletion of ribosomal RNA
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4
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NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, USA)
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Fragmentation of mRNA
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5
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Ion Total RNA Sequencing Kit v2 (Life technologies, USA)
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Adaptor ligation and synthesis of cDNA library
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6
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High Sensitivity DNA Chip Kit (Agilent Bioanalyzer 2100)
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Quantification and size distribution analysis of cDNA library
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7
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Ion Shear Plus Reagents Kit (Life technologies, USA)
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Fragmentation of DNA
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8
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Agencourt Ampure XP DNA Reagent
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Purification and size selection of adaptor-DNA constructs
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9
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QIAamp DNA Mini and Blood Mini Kit (Qiagen, Germany)
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Isolation of DNA from CSF/serum/plasma samples
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10
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Allprep DNA/RNA Mini Kit (Qiagen, Germany)
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Isolation of DNA from tissue samples
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11
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Applied BioSystems TaqMan 2X Universal PCR Master Mix (Invitrogen, USA)
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Real-time PCR for the detection and quantitation of PARV4 DNA
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12
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BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, USA)
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Sequencing of PCR products
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13
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ABI Prism 3100 Genetic Analyzer (Applied Biosystems, USA)
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Automatic sequencing of PCR products
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14
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The Scrub Typhus Detect IgM and IgG ELISAs (InBios International, Inc, Seattle, USA)
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Detection of IgM and IgG antibodies against Orientia tsutsugamushi
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