ParvoDB ID PV016920 GenBank Accession DQ672593
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 245
Definition Human bocavirus strain ViPi-EM NS1 gene, partial cds. Submission Date 2006-06-05
Strain Name ViPi-EM Sampling Date from 2000-01 to 2006-05
Sampling Country Italy Location Italy
Submitted By Andreoli,E., Bendinelli,M., Del Bono,L., Maggi,F., Meschi,S., Pistello,M., Rocchi,J., Vatteroni,M. and Pifferi,M. Submitting Institution Experimental Pathology, University of Pisa, Via San Zeno, 35, Pisa, PI 56127, Italy
Original Host Homo sapiens Sample Type respiratory specimens and nasal swabs
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Nasal swabs To collect specimens from infants, adults, and healthy children. 17336143 View Related Publication
2 Bronchoalveolar lavage (BAL) To collect specimens from adults with severe respiratory diseases.
3 Adenovirus antibody For direct immunofluorescence testing of respiratory viruses.
4 Cytomegalovirus antibody For direct immunofluorescence testing of respiratory viruses.
5 Influenza A and B antibodies For direct immunofluorescence testing of respiratory viruses.
6 Parainfluenza 1-3 antibodies For direct immunofluorescence testing of respiratory viruses.
7 RSV antibodies For both direct immunofluorescence and enzyme immunoassay testing of respiratory syncytial virus.
8 Antibodies for rapid culture in shell vials For the rapid culture of various respiratory viruses.
9 In-house PCR assays For testing rhinoviruses and human metapneumovirus (hMPV).
10 hBoV01.2 primer Sense primer for hBoV PCR amplification.
11 hBoV02.2 primer Antisense primer for hBoV PCR amplification.
12 Ethidium bromide For staining agarose gels to visualize PCR-amplified products.
13 Molecular size markers To compare the size of PCR-amplified products.
14 Big Dye Terminator Cycle Sequencing kit For sequencing the PCR amplicons.
15 Automatic DNA sequencer (Applied Biosystems) For carrying out DNA sequencing.
16 Rotavirus antigen For the antigen testing of fecal specimens.
17 Adenovirus antigen For the antigen testing of fecal specimens.
18 Astrovirus antigen For the antigen testing of fecal specimens.
19 Calicivirus 1 and 2 antigens For the antigen testing of fecal specimens.