ParvoDB ID PV016659 GenBank Accession JQ918261
Subfamily Parvovirinae Genus Protoparvovirus
Species Protoparvovirus primate1 Sequence Length 4912
Definition Bufavirus-1 strain BF.96 NS1, putative VP1, hypothetical protein, and VP2 genes, complete cds. Submission Date 2012-04-11
Strain Name BF.96 Sampling Date 2009
Sampling Country Burkina Faso Location Ouagadougou, Burkina Faso
Submitted By Phan,T., Vo,N., Bonkoungou,I., Kapoor,A., Barro,N., O'Ryan,M., Kapusinszky,B., Wang,C. and Delwart,E. Submitting Institution Virology, Blood Systems Research Institute, 270 Masonic Avenue, San Francisco, CA 94118, USA
Original Host Homo sapiens Sample Type fecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 SD Bioline Rota/Adeno Used for immunochromatographic assay to test for rotavirus. 22855485 View Related Publication
2 Nuclease treatment Used to digest non-particle-protected nucleic acids during viral particle enrichment.
3 Primers with randomized 3' ends Used for nonspecific RNA and DNA amplification in random RT-PCR.
4 Primers BF.F1 and BR.R1 Used for the first round of PCR to detect bufavirus.
5 Primers BF.F2 and BF.R2 Used for nested PCR round to detect bufavirus with an expected amplicon of 440 bp.