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1
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0.45-μm filter (Millipore, Darmstadt, Germany)
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To remove bacterium-sized particles from the fecal supernatants.
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25340816
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2
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Turbo DNase (Ambion, Carlsbad, CA, USA)
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To digest unprotected nucleic acids in the filtrates.
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3
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Baseline-ZERO (Epicenter, Madison, WI, USA)
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To digest unprotected nucleic acids in the filtrates.
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4
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Benzonase (Novagen, San Diego, CA, USA)
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To digest unprotected nucleic acids in the filtrates.
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5
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RNase (Fermentas, Pittsburgh, PA, USA)
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To digest unprotected nucleic acids in the filtrates.
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6
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ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicenter)
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To extract and amplify enriched viral nucleic acids (RNA and DNA).
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7
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RACE (Rapid Amplification of cDNA Ends, Life Technologies)
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To amplify the 5′ and 3′ extremities of the near complete parvovirus genome.
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8
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Nested PCR primers: Tusa-F1, Tusa-R1, Tusa-F2, Tusa-R2
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To target NS1 and determine the prevalence of the virus in the diarrhea samples.
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