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1
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0.45-mm filter (Millipore)
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To remove bacterium-sized particles from fecal supernatants.
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27393975
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2
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DNase and RNase
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To digest unprotected nucleic acids.
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3
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Maxwell 16 automated extractor (Promega)
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To extract nucleic acids.
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4
|
Nextera XT Sample Preparation Kit (Illumina)
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To construct DNA libraries.
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5
|
RACE
|
To amplify the extremities of the genome.
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6
|
MyTaq Extract-PCR Kit (Bioline)
|
To extract nucleic acids from feces and skin biopsies.
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7
|
Superfrost Plus slides (Fisher Scientific)
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To mount formalin-fixed, paraffin-embedded biopsy tissue sections for in situ hybridization.
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8
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RNAscope 2.0 BROWN assay kit (Advanced Cell Diagnostics, Inc.)
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For in situ hybridization.
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|
9
|
3,3′-diaminobenzidine (Advanced Cell Diagnostics, Inc.)
|
To visualize the development of hybridization.
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|
10
|
Hematoxylin
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To counterstain slides.
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11
|
SHUR/Mount (Triangle Biomedical Sciences)
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To mount slides with xylene-based medium.
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