Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV016639 GenBank Accession KT868812
Subfamily Parvovirinae Genus Protoparvovirus
Species Protoparvovirus primate3 Sequence Length 3983
Definition Cutavirus strain BR-450 NS1 gene, partial cds; and putative VP1, hypothetical protein, VP2, and hypothetical protein genes, complete cds. Submission Date 2015-10-02
Strain Name BR-450 Sampling Date
Sampling Country Brazil Location Brazil
Submitted By Phan,T. and Delwart,E. Submitting Institution Virology, Blood Systems Research Institute, 270 Masonic Avenue, San Francisco, CA 94118, USA
Original Host Homo sapiens Sample Type fecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 0.45-mm filter (Millipore) To remove bacterium-sized particles from fecal supernatants. 27393975 View Related Publication
2 DNase and RNase To digest unprotected nucleic acids.
3 Maxwell 16 automated extractor (Promega) To extract nucleic acids.
4 Nextera XT Sample Preparation Kit (Illumina) To construct DNA libraries.
5 RACE To amplify the extremities of the genome.
6 MyTaq Extract-PCR Kit (Bioline) To extract nucleic acids from feces and skin biopsies.
7 Superfrost Plus slides (Fisher Scientific) To mount formalin-fixed, paraffin-embedded biopsy tissue sections for in situ hybridization.
8 RNAscope 2.0 BROWN assay kit (Advanced Cell Diagnostics, Inc.) For in situ hybridization.
9 3,3′-diaminobenzidine (Advanced Cell Diagnostics, Inc.) To visualize the development of hybridization.
10 Hematoxylin To counterstain slides.
11 SHUR/Mount (Triangle Biomedical Sciences) To mount slides with xylene-based medium.