ParvoDB ID PV016628 GenBank Accession GU563342
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 671
Definition Human bocavirus strain JP-8583 VP1 and VP2 genes, partial cds. Submission Date 2010-01-23
Strain Name JP-8583 Sampling Date 2008-05
Sampling Country Japan Location Japan
Submitted By Pham,N.T.K. and Ushijima,H. Submitting Institution Developmental Medical Sciences, International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Original Host Homo sapiens Sample Type fecal specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp viral RNA Mini kit (Qiagen, Inc., GmbH, Hilden, Germany) To extract nucleic acid from fecal suspension samples. 21181924 View Related Publication
2 Primers 188F and 542R To amplify a 354-bp PCR product of the NP-1 region for HBoV detection.
3 Primers VP1/VP2F and VP1/VP2R To amplify an 819-bp segment of the VP1/VP2 gene for HBoV genotyping.
4 BigDye Terminator Cycle Sequencing kit (Perkin Elmer-Applied Biosystems, Inc., Foster City, CA) For sequencing PCR amplicons of the NP-1 and VP1/VP2 genes.