Used with BioRobot M48 for extracting viral DNA and RNA.
3
M-MuLV reverse transcriptase (Promega, Madison, WI, USA)
For obtaining cDNA from RNA.
4
Random primers
Used with M-MuLV reverse transcriptase to synthesize cDNA.
5
Primers derived from the NP1 gene
For performing PCR to detect HBoV initially.
6
Primers derived from the VP1 gene
Used for confirming HBoV detection by a second PCR assay.
7
Standard culture methods
For examining fecal specimens for various intestinal pathogens such as Shigella spp., Salmonella spp., Yersinia enterocolitica, Campylobacter spp., and enteroinvasive Escherichia coli O157.
8
Enzyme immunoassay
For investigating Rotavirus in fecal specimens.
9
Reverse transcriptase PCR
For investigating Norovirus in fecal specimens.
10
Strain Spain001 (GenBank accession no. EF186830)
Used as positive control in each PCR run for HBoV detection.
