Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV016370 GenBank Accession EU078168
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 1629
Definition Human bocavirus VP2 gene, complete cds. Submission Date 2007-08-01
Strain Name None Sampling Date
Sampling Country Thailand Location Thailand
Submitted By Peret,T.C.T. and Erdman,D.D. Submitting Institution GRVLB, CDC, 1600 Clifton Rd MS G-04, Atlanta, GA 30333, USA
Original Host None Sample Type nasopharyngeal swab specimen
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Grace's medium Used for growing Sf9 cells in the production of recombinant baculovirus expressing HBoV VP2 protein. https://patents.google.com/patent/WO2009151697A2/en View Related Publication
2 Fetal Calf Serum (FCS) Added to Grace's medium to support cell growth in Sf9 cell culture.
3 Tris Buffered Saline Used for suspending harvested cells and for lysate preparation.
4 Sucrose Used for creating a sucrose shelf in Tris buffered saline for virus-like particle concentration by centrifugation.
5 Cesium chloride Used for creating a gradient for centrifugation to isolate virus-like particles.
6 DNase I Used for treating isolated bands to degrade any contaminating DNA.
7 1% aqueous uranyl acetate solution Used for staining samples for electron microscopy examination.
8 Phosphotungstic acid Used for negative staining electron microscopy examination.
9 Methanol/acetic acid (70/30) Used for fixing cells in immunofluorescence assays.
10 Normal goat serum Used for blocking in immunofluorescence assays to prevent non-specific binding.
11 Alexa Fluor 488-conjugated goat anti-mouse IgG IgM Used for detecting mouse antibodies in immunofluorescence assays.
12 Fluorescein isothiocyanate goat anti-human IgA+IgG+IgM (H+L) Used for detecting human antibodies in immunofluorescence assays.
13 TMB (tetramethylbenzidine) Used as a substrate in enzyme immunoassays to detect the presence of peroxidase activity.
14 Formvar-carbon grids Used for preparing samples for electron microscopy.
15 Gold conjugated anti-human IgG antibody Used in immunoelectron microscopy for detecting complexes formed with human serum antibodies.
16 Blocking buffer Used to block unoccupied binding sites in enzyme immunoassays.