Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV016219 GenBank Accession DQ234778
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 4865
Definition Human parvovirus B19 isolate R0693 pretransfusion NS1, 7.5 kDa protein, VP1, X protein, VP2, and 11 kDa protein genes, complete cds. Submission Date 2006-06-09
Strain Name R0693 pretransfusion Sampling Date from 2003-11 to 2005-08
Sampling Country Ghana Location Kumasi, Ghana
Submitted By Parsyan,A.E., Szmaragd,C., Allain,J.P. and Candotti,D. Submitting Institution Haematology, University of Cambridge, Cambridge Blood Centre, Long Road, Cambridge, Cambridgeshire CB2 2PT, United Kingdom
Original Host None Sample Type plasma samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Human plasma in EDTA Collected from participants for testing reactivity with genotype-specific recombinant proteins and antibody detection. 16597864 View Related Publication
2 Citrated plasma samples Collected from Ghanaian adult blood donors for testing reactivity with genotype-specific recombinant proteins.
3 Expand High FidelityPLUS PCR system (Roche) Used to perform PCR amplification of VP1 and VP2 regions for expression of genotype 3 proteins.
4 Spodoptera frugiperda (Sf9) cells Grown in SF-900 II medium for producing recombinant proteins using baculovirus expression system.
5 Baculovirus transfer vector pBlueBac4.5/V5-His-TOPO (Invitrogen) Used for cloning VP1 and VP2 coding regions for recombinant baculovirus preparation.
6 High Pure viral nucleic acid kit (Roche) Used for isolating DNA from clinical samples.
7 Transmission Electron Microscopy (Philips CM 100) Used to visualize capsid structures formed by recombinant proteins.
8 Chemicon mouse monoclonal antibodies against VP2 Used for Western blotting to verify the identity of extracted proteins.
9 Biotrin International parvovirus B19 EIAs (commercial kit) Used to test for IgM and IgG antibodies to B19.
10 Proteins VP1-2 from MedImmune Used as part of the genotype 1 capture antigen in enzyme immunoassays.
11 Polymerase Chain Reaction (PCR) reagents Used for genotyping B19 DNA and verifying the amplified sequences.
12 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) Used for protein concentration analysis and purity assessment.
13 QIAquick PCR purification kit (QIAGEN) Used to purify DNA and recombinant plasmids.
14 Negative staining with 2% phosphotungstic acid Used for electron microscopy to visualize recombinant virus-like particles.
15 Monoclonal antibodies against human parvovirus B19 capsid proteins Used to test for presence of specific antibodies in plasma samples.
16 Viral DNA sequencing reagents Used for sequencing B19 DNA to determine genotypes.
17 Phylogenetic analysis software (PAUP 4.0b10) Used for the analysis of genetic distances and constructing phylogenetic trees.
18 WHO International Standard for Anti-Parvovirus B19 plasma (NIBSC code 01/602) Used to calibrate the enzyme immunoassay cutoff levels.