Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV016187 GenBank Accession DQ234773
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 934
Definition Human parvovirus B19 isolate R0227 posttransfusion 1 NS1 and VP1 genes, partial cds. Submission Date 2005-10-11
Strain Name R0227 posttransfusion 1 Sampling Date from 2003-11 to 2005-03
Sampling Country Ghana Location Kumasi, Ghana
Submitted By Parsyan,A.E., Addo-Yobo,E., Owusu-Ofori,S., Akpene,H., Sarkodie,F. and Allain,J.-P. Submitting Institution Haematology, University of Cambridge, Cambridge Blood Centre, Long Road, Cambridge CB2 2PT, United Kingdom
Original Host None Sample Type blood sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Phosphate-buffered saline (PBS) Used for washing steps in enzyme immunoassays and Western blotting 16965589 View Related Publication
2 Tween 20 Included at 0.05 percent in PBS for washing steps to reduce non-specific binding
3 B19 IgM enzyme immunoassay (EIA) kit Used for testing IgM antibodies to B19 in donors and recipients
4 Carbonate-bicarbonate buffer Used for coating ELISA plates with VP2 protein
5 MaxiSorp plates ELISA plates used for coating with VP2 protein
6 Bovine serum albumin (BSA) Used to block non-specific binding sites in ELISA plates and for diluting plasma samples
7 Rabbit anti-human IgG-horseradish peroxidase conjugate Secondary antibody used in ELISA detection
8 1-Step Ultra TMB-ELISA substrate Substrate used in ELISA to produce a colorimetric reaction
9 H2SO4 Sulfuric acid used to stop the ELISA reaction
10 Tris-buffered saline Used for washing steps in Western blotting
11 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) materials Used for separating proteins under denaturing conditions in Western blotting
12 Nitrocellulose membrane Membrane used to transfer proteins for Western blotting
13 Blocking buffer Used to block non-specific sites on the nitrocellulose membrane in Western blotting
14 Goat anti-human IgG Fc fragment antibodies conjugated to alkaline phosphatase Secondary antibody used in Western blotting for detection
15 5-Bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium Substrate used in Western blotting to visualize protein bands