Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV016040 | GenBank Accession | GU951564 |
| Subfamily | Parvovirinae | Genus | Tetraparvovirus |
| Species | Tetraparvovirus primate1 | Sequence Length | 1457 |
| Definition | Human parvovirus 4 strain Ghana19 ORF1 and ORF2 genes, partial cds. | Submission Date | 2010-03-02 |
| Strain Name | Ghana19 | Sampling Date | 2005 |
| Sampling Country | Ghana | Location | villages in the rural Afigya Sekyere district, Ghana |
| Submitted By | Panning,M., Kobbe,R., Vollbach,S., Drexler,J.F., Adjei,S., Adjei,O., Drosten,C., May,J. and Eis-Hubinger,A.M. | Submitting Institution | Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, Bonn 53105, Germany |
| Original Host | Homo sapiens | Sample Type | blood samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | EDTA | For storage under tropical conditions, an equal volume of whole blood in EDTA was supplemented with buffer AS1. | 20587191 | View Related Publication |
| 2 | Buffer AS1 (QIAGEN, Hilden, Germany) | Supplemented with whole blood in EDTA for storage under tropical conditions. | ||
| 3 | MagAttract M48 DNA Mini Kit (QIAGEN) | Used for DNA isolation on a BioRobot M48 workstation as recommended. | ||
| 4 | Primers 5′-ACCAAGGACACCAGACAGTCTT-3′ and 5′-ACGTGTTCAGACCAAAAGGAT-3′ | Developed for specific real-time PCR to detect novel PARV4 genotype 3. | ||
| 5 | Probe 5′-FAM-CCAGCTCCATACCTTTCAGCAGTTGC-BHQ1-3′ | Used in the real-time PCR assay for detecting PARV4 genotype 3. | ||
| 6 | Plasmid-based standard derived from the real-time PCR amplicon of sample Ghana19 | Used for absolute quantification of genome copy numbers in PCR retesting of all samples. |