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1
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Peripheral Blood Mononuclear Cells (PBMC)
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Sample used in the study, composed of cells collected from calves.
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34960693
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2
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Phosphate Buffered Saline (PBS)
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Dilution of the PBMC pooled sample.
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3
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Centrifuge
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To centrifuge commercial batches of bovine serum and PBMC samples for clarification.
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4
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0.45 μm Pore Size Membrane (Millipore)
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Filtration of the supernatants of bovine serum and PBMC samples.
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5
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Sucrose Cushion (25%)
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Used in ultracentrifugation to separate viral particles.
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6
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Sorvall AH629 Rotor (Thermo Fisher Scientific)
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Equipment used for ultracentrifugation of the samples.
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7
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DNase and RNase Enzymes (Thermo Fisher Scientific)
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Incubation with the viral particle pellet to degrade free nucleic acids.
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8
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TRIzol LS Reagent (Thermo Fisher Scientific)
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Isolation of viral RNA from the samples.
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9
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Phenol-Chloroform
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Extraction method for viral DNA.
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10
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GenomePlex Complete Whole Genome Amplification (WGA) Kit (Sigma-Aldrich)
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Multiple displacement amplification (MDA) for viral DNA enrichment.
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11
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SeqPlex RNA Amplification Kit (Sigma-Aldrich)
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Reverse transcription and subsequent RNA enrichment.
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12
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PureLink Quick Gel Extraction and PCR Purification Combo Kit (Thermo Fisher Scientific)
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Purification of enriched DNA products.
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13
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NanoDrop (Thermo Fisher Scientific)
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Spectrophotometry for assessment of DNA quality and quantity.
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14
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Qubit 4 (Thermo Fisher Scientific)
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Fluorometry for measuring DNA concentration.
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15
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Nextera XT DNA Library Preparation Kit
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Preparation of DNA libraries for sequencing.
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16
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Illumina iSeq 100 System and Illumina i1 Reagent Kit
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High-throughput sequencing process.
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