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1
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Third-generation enzyme-linked immunosorbent assay (ELISA) kits (Human Diagnostics, Wiesbaden, Germany)
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Detection of HBsAg and anti-HCV antibodies in serum samples.
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21328387
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2
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Parvovirus B19 IgM and IgG enzyme immunoassay Kits (Parvovirus B19 IgG and IgM, DxSelectTM FOCUS Diagnostic, Hannover, Germany)
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Detection of antibodies to human parvovirus B19.
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3
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QIA Amp blood kit (QIAgen, Hilden, Germany)
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DNA extraction from serum samples.
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4
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Outer primer pair HBPr134 (sense) and HBPr135 (antisense)
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Initial PCR amplification targeting highly conserved regions of the S-gene of HBV.
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5
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10X PCR buffer (Qiagen)
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Providing the necessary chemical environment for DNA polymerase activity during PCR.
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6
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dNTPs
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Nucleotide substrates for DNA synthesis during PCR.
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7
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Ampli Taq gold DNA polymerase (Applied Biosystems, Foster City, CA)
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Catalyzing the synthesis of DNA during PCR.
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8
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Inner primer pair HBPr75 (sense) and HBPr94 (antisense)
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Nested PCR amplification targeting HBV for increased specificity.
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9
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Exo-SAP-IT (exonuclease I and shrimp alkaline phosphatase enzyme, USB, Cleveland, OH)
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Purification of PCR products before sequencing.
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10
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Forward and reverse primers (nP5R and nP5F)
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Sequencing of the NS1/VP1u region of parvovirus B19 DNA.
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11
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Dye labeled dideoxy terminators (Big Dye, Applied Biosystems)
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Performing the Sanger sequencing reaction.
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