Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV015561 | GenBank Accession | KR011997 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 771 |
| Definition | Human bocavirus isolate 308828HBoV/PAN/11 structural protein VP1/VP2 gene, partial cds. | Submission Date | 2015-03-25 |
| Strain Name | 308828HBoV/PAN/11 | Sampling Date | 2011-03-23 |
| Sampling Country | Panama | Location | Panama |
| Submitted By | Moreno,B., Abrego,L., Carrera,J.P., Franco,D., Gaitan,M., Castillo,J., Pascale,J. and Arbiza,J. | Submitting Institution | Investigacion en Virologia y Biotecnologia, Instituto Conmemorativo Gorgas de Estudios de la Salud, Ave. Justo Arosemena, entre calle 35 y 36, Panama 0816-0293, Panama |
| Original Host | Homo sapiens | Sample Type | Nasopharyngeal swabs |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp RNA viral extraction kit | Extraction of viral RNA | 26252655 | View Related Publication |
| 2 | DNase I | Treatment of samples to remove any DNA | ||
| 3 | QIAGEN OneStep RT-PCR kit | Amplification of HBoV NP-1 gene | ||
| 4 | Primers (previously described by Allander et al., 2005) | Amplification of a 354 bp fragment of the HBoV NP-1 gene | ||
| 5 | Qiagen DNeasy Blood & Tissue DNA extraction Kit | DNA extractions to detect Adenovirus (ADV) infection | ||
| 6 | 7500 Fast thermocycler (Applied Biosystems, Foster City, CA) | Real-time RT-PCR reactions for other respiratory viruses (Flu A, Flu B, RSV, P1, P2, P3, RV, Metapneumovirus) | ||
| 7 | Primers (described by Bastien et al.) | Amplification of VP1/VP2 genes for assessment of viral diversity | ||
| 8 | Qiaquick PCR purification Kit (Qiagen, Germany) | Purification of PCR amplicons | ||
| 9 | ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit | Sequencing of PCR amplicons | ||
| 10 | 3130XL Genetic Analyzer (Applied Biosystems, Foster City, CA) | Conducting sequencing reactions |