Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV015430 GenBank Accession JN211169
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 4280
Definition Human parvovirus B19 isolate 181246 non-structural protein 1 (NS1), VP1 protein (VP1), and VP2 protein (VP2) genes, partial cds. Submission Date 2011-07-05
Strain Name 181246 Sampling Date 2006-03
Sampling Country Netherlands Location Netherlands
Submitted By Molenaar-de Backer,M.W.A., van Swieten,P. and Zaaijer,H.L. Submitting Institution Blood-Borne Infections, Sanquin Blood Supply Foundation, Plesmanlaan 125, Amsterdam 1066CX, The Netherlands
Original Host Homo sapiens Sample Type sera samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Roche LightCycler parvovirus B19 quantification assay Testing blood donations for presence of B19V DNA 22912828 View Related Publication
2 NucliSENS EasyMag extractor (BioMerieux) Extraction of B19V DNA from donor samples
3 NSoFw and Vp2oRev primers Primers for almost full genome PCR in B19V DNA amplification
4 2 units of long range PCR enzyme Enzyme component in PCR mixture for DNA amplification
5 500 mM of each deoxyribonucleotide triphosphate (dNTP) Component in PCR mixture for DNA amplification
6 1x Long range buffer containing 2.5 mM MgCl2 (Qiagen) Buffer component in PCR mixture for DNA amplification
7 ExoSAP-IT (USB) Purification of PCR products for sequencing reactions
8 Big Dye Terminator cycle sequencing (Applied Biosystems) Sequencing reactions of purified PCR products
9 B19V specific sequencing primers Primers for B19V sequencing reactions
10 ABI PRISM 3130xl Genetic Analyser (Applied Biosystems) Performing sequence analysis of B19V strains