Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV015331 | GenBank Accession | OP751140 |
| Subfamily | Parvovirinae | Genus | Protoparvovirus |
| Species | Protoparvovirus primate3 | Sequence Length | 216 |
| Definition | Cutavirus isolate AS2_230 capsid protein (VP2) gene, partial cds. | Submission Date | 2022-11-01 |
| Strain Name | AS2_230 | Sampling Date | 2014 |
| Sampling Country | Finland | Location | Finland |
| Submitted By | Mohanraj,U., Konttinen,T., Salava,A., Vakeva,L., Ranki,A. and Soderlund-Venermo,M. | Submitting Institution | Department of Virology, University of Helsinki, E358, Haartmaninkatu 3, Helsinki 00290, Finland |
| Original Host | Homo sapiens | Sample Type | Skin samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Formalin | Used for fixing skin biopsy samples | 37234048 | View Related Publication |
| 2 | Paraffin | Used for embedding skin biopsy samples | ||
| 3 | CuV probes targeting the virus protein (VP) region | Used for RNAscope in situ hybridization (RISH) to detect CuV DNA in skin biopsies | ||
| 4 | Human gene PPIB probe | Served as a positive technical control for RISH | ||
| 5 | Bacterial gene DapB probe | Served as a negative technical control for RISH | ||
| 6 | Insect cells infected with CuV VP-bearing baculovirus | Served as positive CuV-probe control for RISH | ||
| 7 | Insect cells noninfected with CuV VP-bearing baculovirus | Served as negative CuV-probe control for RISH | ||
| 8 | Primers and probes for multiplex real-time quantitative PCR | Used to detect and quantify DNA of CuV, BuV, and TuV | ||
| 9 | Non-template controls | Included in all PCR runs as controls | ||
| 10 | RNaseP qPCR | Used as a quality control to ensure that DNA was intact |