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1
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Nasopharyngeal swabs
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Collected from children with symptoms of respiratory tract infection.
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31146533
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2
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Cold boxes (2–8 °C)
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Transport of nasopharyngeal specimens to the virology laboratory.
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3
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Phosphate buffered saline (PBS)
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Used to dilute the mucus in the respiratory samples.
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4
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Acetone
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Fixation of respiratory samples on slides for immunofluorescence staining.
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5
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Euroimmun RSV commercial kit (USA)
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Detection of RSV antigens in respiratory samples using direct immunofluorescence technique.
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6
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Conjugated purified murine monoclonal antibodies
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Staining against the fusion protein and nucleoprotein of RSV.
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7
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Viral nucleic acid extraction kit (DynaBio, Iran)
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Extraction of viral nucleic acids from respiratory secretion.
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8
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Primers for NP-1 coding region (F: 5′-AGCTCTGTAAGTACTATTAC-3′ and R: 5′-CTCTGTGTTGACTGAATA-CAG-3′)
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PCR-based detection of HBoV DNA.
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9
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SYBR Safe DNA gel stain (ThermoFisher Scientific, USA)
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Staining of agarose gel for visualization of PCR products.
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10
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Cloned plasmid pTZ containing HBoV NP-1 gene (Invitrogen, Carlsbad, CA, USA)
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Used as positive control for PCR.
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11
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Forward primer (5′-GGACCACAGTCATCAGAC-3′) and reverse primer (5′-CCACTACCATCGGGCTG-3′)
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Amplification of 820 bp of the HBoV VP2 gene for genotyping.
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