Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV015269 GenBank Accession MH061315
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 325
Definition Human bocavirus strain 329641 nonstructural protein 1 gene, partial cds. Submission Date 2018-03-13
Strain Name 329641 Sampling Date 2018-02-11
Sampling Country Iran Location Imam Hussein Central Pediatric Hospital, Iran
Submitted By Mohammadi,M., Moghim,D., Karbasizadeh,D. and Yavarian,D. Submitting Institution Isfahan University of Mefical Science, Department of Medical Microbiology and Virology, Azadi Aquare-Hezar Jerib Street, Isfahan, Isfahan 8583115961, Iran
Original Host Homo sapiens Sample Type Nasopharyngeal swabs
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Nasopharyngeal swabs Collected from children with symptoms of respiratory tract infection. 31146533 View Related Publication
2 Cold boxes (2–8 °C) Transport of nasopharyngeal specimens to the virology laboratory.
3 Phosphate buffered saline (PBS) Used to dilute the mucus in the respiratory samples.
4 Acetone Fixation of respiratory samples on slides for immunofluorescence staining.
5 Euroimmun RSV commercial kit (USA) Detection of RSV antigens in respiratory samples using direct immunofluorescence technique.
6 Conjugated purified murine monoclonal antibodies Staining against the fusion protein and nucleoprotein of RSV.
7 Viral nucleic acid extraction kit (DynaBio, Iran) Extraction of viral nucleic acids from respiratory secretion.
8 Primers for NP-1 coding region (F: 5′-AGCTCTGTAAGTACTATTAC-3′ and R: 5′-CTCTGTGTTGACTGAATA-CAG-3′) PCR-based detection of HBoV DNA.
9 SYBR Safe DNA gel stain (ThermoFisher Scientific, USA) Staining of agarose gel for visualization of PCR products.
10 Cloned plasmid pTZ containing HBoV NP-1 gene (Invitrogen, Carlsbad, CA, USA) Used as positive control for PCR.
11 Forward primer (5′-GGACCACAGTCATCAGAC-3′) and reverse primer (5′-CCACTACCATCGGGCTG-3′) Amplification of 820 bp of the HBoV VP2 gene for genotyping.