Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV015212 GenBank Accession AF293863
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 750
Definition B19 virus isolate 2_980728 VP1 protein gene, partial cds. Submission Date 2000-08-07
Strain Name 2_980728 Sampling Date
Sampling Country None Location None
Submitted By Modrow,S. Submitting Institution Institut fuer Medizinische Mikrobiologie und Hygiene, Franz-Josef-Strauss Allee 11, Regensburg 93053, Germany
Original Host Homo sapiens Sample Type Serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 His-tagged protein Used for immunizing rabbits to generate polyclonal antisera directed against the VP1-unique region. 11125172 View Related Publication
2 Ni2+-affinity chromatography Used for purifying His-tagged protein expressed in E. coli.
3 Culture supernatants Source for purifying human monoclonal antibodies (MAbs) against the VP1-unique region and VP2 virus-like particles using protein G-affinity chromatography.
4 Chitin-binding domain (CBD) Part of the heterologous construct fused with intein for purifying the recombinant VP1-unique region.
5 Chitin column Used for affinity chromatography to purify recombinant proteins fused with intein and CBD.
6 Dithiothreitol (DTT) Used as a reducing agent to elute the VP1-unique region from the intein-CBD construct during protein purification.
7 Centriplus concentrator (3 kDa exclusion limit; Amicon) Used for concentrating the pooled and purified VP1-unique protein fractions.
8 Bradford assay (Bio-Rad Laboratories) Used to determine protein concentration after dialysis against PBS.
9 Caesium chloride gradient centrifugation Used for purifying recombinant VP2 capsids and VP1/VP2 capsids produced via recombinant baculovirus.
10 Nitrocellulose membrane Used for transferring proteins during Western blot assays.
11 5% low-fat dried milk in Tris-buffered saline Used to block protein-free regions of nitrocellulose membrane during Western blot assays.
12 Alkaline phosphatase-conjugated secondary antibodies Used to detect primary antibodies in Western blot assays (specifically secondary antibodies targeting rabbit and human IgG).
13 Quartz crystals with gold electrodes Used in the quartz crystal microbalance biosensor system to quantify specific antibody-antigen interactions.
14 Dithiobissuccinimidyl propionate Used to activate gold electrodes for binding protein layers in the quartz crystal microbalance biosensor.
15 Protein A Used to coat quartz crystals and facilitate binding of human monoclonal antibodies.
16 Bovine serum albumin (BSA) Used as a blocking reagent to prevent non-specific binding in quartz crystal microbalance biosensor assays.