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1
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LLC-MK2 cell line
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To culture clinical specimens.
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24518633
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2
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HEp-2 cell line
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To culture clinical specimens.
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3
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Rhabdomyosarcoma (RD) cell line
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To culture clinical specimens.
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4
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MDCK cell line
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To culture clinical specimens.
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5
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Dubelcco’s modified eagle medium
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To supplement culture media for cell lines.
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6
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2% fetal bovine serum
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To supplement culture media for cell lines.
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7
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1% antibiotic
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To supplement culture media for cell lines.
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8
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Qiagen QIAmp DNA blood mini kit
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To extract DNA from samples.
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9
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dNTPs mix
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To provide nucleotides for the PCR reaction.
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10
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Taq polymerase
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To catalyze DNA synthesis during PCR.
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11
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Ethidium bromide
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To stain DNA for visualization in agarose gel electrophoresis.
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12
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1.5% (w/v) agarose
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To create gels for electrophoresis.
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13
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1X Tris Borate EDTA buffer pH 8.0
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To run the electrophoresis gels.
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14
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100 bp and 1 Kb DNA ladder (Fermentas)
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To serve as size references in electrophoresis.
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15
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Qiagen QIAquick PCR purification kit
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To purify PCR products before sequencing.
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16
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Thermo Scientific NanoDrop 2000/2000c spectrophotometer
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To determine the concentration and purity of DNA.
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17
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Gene Amp 9600 themocycler (Applied Biosystems)
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To perform cycle sequencing.
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18
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Centri–SepTM columns
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To remove excess deoxy terminator from DNA sequencing products.
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19
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ABI Prism 3100 Genetic analyzer version 2 (Applied Biosystems)
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To perform automated sequencing.
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20
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ABI Prism BigDye Terminator cycle ready reaction kit version 2.0
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To prepare DNA for sequencing.
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