Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV014956 GenBank Accession EU487018
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 132
Definition Human parvovirus 4 isolate B22238 ORF1 gene, partial cds. Submission Date 2008-02-13
Strain Name B22238 Sampling Date 2000
Sampling Country Thailand Location Thailand
Submitted By Lurchachaiwong,W., Chieochansin,T., Payungporn,S., Theamboonlers,A. and Poovorawan,Y. Submitting Institution Department of Pediatrics, Centre of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Bangkok 10330, Thailand
Original Host None Sample Type serum sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 DNA Template for the PCR reaction mixture. 18759058 View Related Publication
2 Sense primer PV4ORF1F Part of the PCR reaction mixture to amplify a 220-bp region of PARV4.
3 Antisense primer PV4ORF1R Part of the PCR reaction mixture to amplify a 220-bp region of PARV4.
4 MasterMix (Eppendorf, Hamburg, Germany) Component of the PCR reaction mixture for amplification.
5 Nuclease-free water Used to bring the PCR reaction mixture to a final volume of 25 μl.
6 Hot-StartTaq DNA polymerase Activated during PCR pre-incubation to initiate the PCR process.
7 Primer PV4ORF1F2 Used for semi-nested amplification to produce a 159-bp fragment.
8 Plasmid Template for PCR sensitivity tests based on concentration determined by OD260.
9 Murex (Abbott Laboratory, Wiesbaden, Germany) Used for serological testing of HIV Ag/Ab combination.
10 HBsAg (AUSAB EIA) Used for serological testing for hepatitis B.
11 AntiHCV (ABBOTT HCV EIA 3.0) Used for serological testing for hepatitis C.