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1
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Ficoll-Paque
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Used for separating mononuclear cells from whole blood cells.
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8337823
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2
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Fetal calf serum (FCS)
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Used for washing and culturing cells.
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3
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Iscove's modification of Dulbecco's medium (IMDM)
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Used as a culture medium for cells.
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4
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Human AB serum
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Used as a supplement in the culture medium.
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5
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Bovine serum albumin (BSA)
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Used as a supplement in the culture medium.
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6
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Penicillin and streptomycin
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Used as antibiotics in the culture medium.
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7
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Human recombinant interleukin 3 (hrIL-3)
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Used to support cell growth in the culture medium.
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8
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Erythropoietin
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Used to support cell growth in the culture medium.
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9
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B19 containing positive serum
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Used to infect cells with B19 virus.
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10
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B19 negative serum
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Used as a mock control in infection experiments.
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11
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DEAE-dextran
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Used for transfection of COS-7 cells.
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12
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Smal and Xbal
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Restriction enzymes used for digestion of plasmid DNA.
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13
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pGEM3Zf(+) vector
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Used as a cloning vector.
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14
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Synthetic oligonucleotide
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Used for in vitro mutagenesis.
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15
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Keyhole limpet hemacyanin (KLH)
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Used for conjugation and immunization of rabbits.
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16
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SP6 RNA polymerase
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Used for in vitro transcription of RNA.
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17
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[35S]-labeled methionine
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Used for labeling proteins in translation reactions.
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18
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[3H]-labeled leucine and proline
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Used for labeling cells in culture.
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19
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RIPA buffer
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Used for immunoprecipitation.
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20
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Protein A-sepharose
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Used for binding and precipitation of antibody-protein complexes.
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21
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SDS-PAGE
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Used for separation of proteins by electrophoresis.
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22
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Enhance (DuPont)
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Used for treating gels before autoradiography.
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23
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Kodak XAR film
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Used for autoradiography.
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