Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV014886 | GenBank Accession | MG953833 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 5157 |
| Definition | Human bocavirus 3 strain HBoV-3/BRA/TO-40/Brazil/2014 isolate BRA/TO-40, complete genome. | Submission Date | 2018-02-14 |
| Strain Name | HBoV-3/BRA/TO-40/Brazil/2014 | Sampling Date | 2014 |
| Sampling Country | Brazil | Location | Tocantins State, Brazil |
| Submitted By | Luchs,A. | Submitting Institution | Enteric Diseases Laboratory - Virology Center, Adolfo Lutz Institute, Av Dr Arnaldo, 355, Sao Paulo, SP 01246-902, Brazil |
| Original Host | Homo sapiens | Sample Type | fecal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Hanks’ buffered salt solution (HBSS) | To dilute human fecal samples. | 29700140 | View Related Publication |
| 2 | Lysing matrix C (MP Biomedicals, USA) | For homogenizing the fecal samples. | ||
| 3 | 0.45-μm-pore-size filter (Merck Millipore, Billerica, MA, USA) | To filter the supernatant after centrifugation. | ||
| 4 | Mixture of nuclease enzymes | To digest unprotected nucleic acids in the filtrates. | ||
| 5 | Zymo Research ZR-96 viral DNA/RNA kit (Irvine, CA, USA) | For viral nucleic acids extraction. | ||
| 6 | Avian myeloblastosis virus (AMV) reverse transcription (Promega, Inc., Madison, WI, USA) | For cDNA synthesis. | ||
| 7 | DNA polymerase I large (Klenow) fragment (Promega, Inc., Madison, WI, USA) | For second-strand cDNA synthesis. | ||
| 8 | Nextera XT sample preparation kit (Illumina, Inc., San Diego, CA, USA) | To construct a DNA library. |