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1
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Viral transport medium (UTM, Copan, Italy)
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Transport and preservation of respiratory specimens collected from patients.
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34578309
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2
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RibospinvRD Kit (GeneAll Biotechnology, Seoul, Korea)
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Isolation of viral DNA and RNA from viral transport medium.
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3
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Seeplex RV15 detection kit (Seegene Inc., Seoul, Korea)
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Multiplex PCR amplification of specific viral nucleic acid from samples.
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4
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MCE-202 MultiNA device (Shimadzu, Kyoto, Japan)
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Detection of PCR products by microchip electrophoresis.
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5
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Multiplex-tandem polymerase chain reaction kit for respiratory viruses (MT-PCR, AusDiagnostics Pty Ltd., Sydney, NSW, Australia)
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Multiplex PCR detection of respiratory viruses, including HBoV.
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6
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Quick-DNA Viral Kit (Zymo Research)
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Re-extraction of viral DNA from HBoV positive samples.
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7
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Phusion polymerase (New England Biolabs)
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PCR amplification of near-complete HBoV genome or fragments.
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8
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1% agarose gels
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Separation of PCR amplification products.
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9
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Nucleospin Gel and PCR Clean-up Kit (Macherey-Nagel, Dueren, Germany)
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Purification of excised PCR products from agarose gels.
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10
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QuantiFluor ONE dsDNA System (Promega, Madison, WI, USA)
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Quantification of purified PCR products.
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11
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NEBNext Ultra II FS DNA Library prep kit (New England Biolabs, Ipswich, Great Britain)
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Preparation of DNA libraries for next-generation sequencing.
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12
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2100 Bioanalyzer and High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA)
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Quality check of prepared DNA libraries.
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13
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MiniSeq Mid Output Kit (Illumina, San Diego, CA, USA)
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Sequencing of DNA libraries to obtain paired-end reads.
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