ParvoDB ID PV014513 GenBank Accession GU338055
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 5299
Definition Human bocavirus strain LWK, complete genome. Submission Date 2009-12-18
Strain Name LWK Sampling Date 2009-11-15
Sampling Country China Location Guangzhou, Guangdong, China
Submitted By Liu,W.K. Submitting Institution GIRD, KangDa Street 1, GuangZhou 510230, China
Original Host Homo sapiens Sample Type Throat swab
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp DNA Mini Kit (Qiagen) DNA extraction from respiratory samples 22168387 View Related Publication
2 Taqman real-time PCR primers and probe (synthesized by TaKaRa) Detection of HBoV DNA based on the NP1 gene
3 Premix Ex Taq (Perfect Real Time) (TaKaRa) Real-time PCR reaction buffer
4 Positive control plasmid inserted into pMD18-T vector (TaKaRa) Quantification analysis for PCR
5 Detection kits for other respiratory pathogens (Guangzhou HuYanSuo Medical Technology Co., Ltd) Testing for other potential pathogens in HBoV positive samples using Taqman real-time PCR
6 PCR amplification primer sets Design based on HBoV genome sequences for complete genome sequencing
7 Pfu DNA polymerase (Fermentas) PCR amplification of DNA template
8 QIAquick Gel Extraction Kit (Qiagen) Purification of PCR products after agarose gel electrophoresis