Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV014393 GenBank Accession KP242037
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 5271
Definition Human bocavirus isolate HRV-HFMD01 NS1 (NS1), NP1 (NP1), VP1 (VP1), and VP2 (VP2) genes, complete cds. Submission Date 2014-12-02
Strain Name HRV-HFMD01 Sampling Date 2012
Sampling Country Thailand Location Thailand
Submitted By Linsuwanon,P., Li,L., Deng,X., Poovorawan,Y. and Delwart,E. Submitting Institution Pediatrics, Chullongkorn University, Patumwan, Bangkok 10330, Thailand
Original Host Homo sapiens Sample Type fecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 1x phosphate buffer saline (PBS) solution Used to suspend stool samples for fecal supernatant preparation. 26288145 View Related Publication
2 Turbo DNase (Life Technologies, NY, USA) Used for digestion to remove non-viral nucleic acids.
3 Baseline-ZERO (Epicentre, WI, USA) Used for digestion to remove non-viral nucleic acids.
4 RNase (Fermentas, Ontario, Canada) Used for digestion to remove non-viral nucleic acids.
5 QIAamp viral RNA extraction kit (Qiagen, Hilden, Germany) Used for the extraction of viral nucleic acids.
6 ScriptSeq RNA-Seq library preparation kit (Epicentre, WI, USA) Used for the preparation of DNA for sequencing.
7 Agencourt AMPure magnetic-bead (Beckmann Coulter, CA, USA) Used for purification of di-tagged single strand cDNAs and adaptor-tagged libraries.
8 KAPA library quantification kit (Kapa Biosystems, MA, USA) Used for quantification of the purified sequencing libraries.