Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV014197 GenBank Accession AJ717293
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 4922
Definition B19 virus NS1 gene, gene for 7.5KDa protein, gene for 11KDa protein, VP1 gene and VP2 gene, isolate Berlin, genotype 2. Submission Date 2004-05-21
Strain Name Berlin Sampling Date
Sampling Country Germany Location Berlin, Germany
Submitted By Liefeldt,L., Plentz,A., Raab,U., Klempa,B., Kershaw,O., Endres,A.S., Neumayer,H.H., Meisel,H. and Modrow,S. Submitting Institution Modrow S., Institute for Medical Microbiology, university of Regensburg, Franz-Josef-Strauss-Allee 11, 93953 Regensburg, GERMANY
Original Host Homo sapiens Sample Type serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Erythropoietin Treatment for anemia secondary to chronic renal failure. 15543575 View Related Publication
2 Cyclosporine Part of the initial triple immunosuppressive regimen.
3 Mycofenolate mofetil Part of the initial triple immunosuppressive regimen.
4 Prednisolone Part of the initial triple immunosuppressive regimen.
5 Tacrolimus Substitute for cyclosporine after an episode of rejection.
6 Packed red cells Transfusion due to deteriorating anemia.
7 High dose intravenous immunoglobulin (IVIG) Treatment for resolving anemia and symptoms due to parvovirus B19 infection.
8 Parvovirus B19 ELISA (Biotrin, Dublin, Ireland) Detection of IgG and IgM against VP2 proteins.
9 RecomBlot assay (Mikrogen GmbH, Munich, Germany) Detection of IgG and IgM against VP1, VP2, and NS1 proteins using Western blot.
10 QIAamp Blood Kit (Qiagen) Isolation of viral nucleic acid from serum samples.
11 GeneAmp PCR System 2400 (Perkin-Elmer Applied Biosystems) Initial qualitative PCR for the detection of parvovirus B19 DNA.
12 Digoxigenin-labelled primers Part of the PCR assay for detecting parvovirus B19/genotype 1.
13 Biotin-labelled oligonucleotide probe Detection of DNA-amplificates in the initial PCR assay.
14 ABI PRISM 7700 Sequence Detection System (Perkin Elmer-Applied Biosystems) Quantitative PCR for amplification of the NS1-encoding region of parvovirus B19.
15 Probes P1, P2, and P3 Detection of parvovirus B19 genotypes 1, 2, and 3 in quantitative PCR.
16 QIAamp DNA Mini Kit (Qiagen) Isolation of viral DNA for cloning.
17 pFastBAC1 (Invitrogen, Karlsruhe, Germany) Vector for cloning the genome segments of parvovirus B19/genotype 2.
18 Serial dilutions of plasmids Determination of the performance and sensitivity of the quantitative PCR for genotypes 2 and 3.
19 Parvovirus B19 international standard 99/800 (National Institute for Biological Standards and Control, Potters Bar, UK) Evaluation and calibration of the quantitative PCR assay.
20 Serial dilutions of purified plasmid DNA Adjusting the sensitivity of the PCR assay for genotypes 2 and 3.