Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV013415 GenBank Accession AY064475
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 4844
Definition Erythrovirus A6 clone c2 non-structural protein, 7.5 kDa protein, capsid protein VP1, and 11 kDa protein genes, complete cds. Submission Date 2001-11-29
Strain Name None Sampling Date from 1991 to 2001
Sampling Country None Location None
Submitted By Nguyen,Q.T., Wong,S. and Brown,K.E. Submitting Institution Hematology Branch, National Heart, Lung, and Blood Institute, 9000 Rockville Pike, Bethesda, MD 20892, USA
Original Host human Sample Type Serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 NS1/7.5EC PCR assay primers Designed from a conserved region of the B19/V9 sequence to identify and differentiate parvovirus variants. 12359439 View Related Publication
2 MfeI restriction enzyme Allows differentiation between B19- and V9-like sequences.
3 DNA extraction kits (DNAEAsy method from Qiagen, Inc.) Used to extract DNA from serum, plasma, bone marrow samples, and plasma pools.
4 Plasmid pYT103 containing the entire coding region of B19-Au isolate Used as a control in PCR and dot blot hybridizations.
5 Plasmid V9-C22 containing the complete coding region of V9 Used as a control in PCR and dot blot hybridizations.
6 Random-primed B19 and V9 32P-labeled probes Used in B19 and V9 dot blot hybridizations to detect parvovirus DNA.
7 ExTaq DNA polymerase (Panvera Corp.) Used in the primary round of B19 PCR.
8 AmpliTaq Gold (Perkin–Elmer) PCR enzyme used in NS1/7.5EC PCR assays.
9 BigDye terminator cycle sequencing kit (Applied Biosystems) Used for sequencing the PCR products.
10 PCR2.1 TOPO (Invitrogen) TA cloning of PCR products.
11 Top10 cells (Invitrogen) Used for transformation following TA cloning.
12 RNA Stat 60 (TelTest) Extraction of RNA from infected cell samples.
13 DNAse I treatment Removal of residual DNA from RNA samples.
14 Superscript II reverse transcriptase (Life Technologies) cDNA synthesis from extracted RNA.
15 Alkaline-phosphatase-labeled probe Confirmation of PCR product specificity in Southern blotting.
16 tRNA Used as a carrier in RNA extraction.
17 Iscove’s modified DMEM with 10% fetal calf serum and 2 U/mL erythropoietin Growth medium for UT7/Epo/S1 cells.
18 B19 IgM and IgG radioimmunoassay Detection of antibodies in the A6 serum sample.