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1
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NS1/7.5EC PCR assay primers
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Designed from a conserved region of the B19/V9 sequence to identify and differentiate parvovirus variants.
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12359439
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View Related Publication
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2
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MfeI restriction enzyme
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Allows differentiation between B19- and V9-like sequences.
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3
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DNA extraction kits (DNAEAsy method from Qiagen, Inc.)
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Used to extract DNA from serum, plasma, bone marrow samples, and plasma pools.
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4
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Plasmid pYT103 containing the entire coding region of B19-Au isolate
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Used as a control in PCR and dot blot hybridizations.
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5
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Plasmid V9-C22 containing the complete coding region of V9
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Used as a control in PCR and dot blot hybridizations.
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6
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Random-primed B19 and V9 32P-labeled probes
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Used in B19 and V9 dot blot hybridizations to detect parvovirus DNA.
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7
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ExTaq DNA polymerase (Panvera Corp.)
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Used in the primary round of B19 PCR.
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8
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AmpliTaq Gold (Perkin–Elmer)
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PCR enzyme used in NS1/7.5EC PCR assays.
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9
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BigDye terminator cycle sequencing kit (Applied Biosystems)
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Used for sequencing the PCR products.
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10
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PCR2.1 TOPO (Invitrogen)
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TA cloning of PCR products.
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11
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Top10 cells (Invitrogen)
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Used for transformation following TA cloning.
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12
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RNA Stat 60 (TelTest)
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Extraction of RNA from infected cell samples.
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13
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DNAse I treatment
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Removal of residual DNA from RNA samples.
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14
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Superscript II reverse transcriptase (Life Technologies)
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cDNA synthesis from extracted RNA.
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15
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Alkaline-phosphatase-labeled probe
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Confirmation of PCR product specificity in Southern blotting.
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16
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tRNA
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Used as a carrier in RNA extraction.
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17
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Iscove’s modified DMEM with 10% fetal calf serum and 2 U/mL erythropoietin
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Growth medium for UT7/Epo/S1 cells.
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18
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B19 IgM and IgG radioimmunoassay
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Detection of antibodies in the A6 serum sample.
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