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1
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Polyethylene glycol (PEG-6000)
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Used for water sample concentration through precipitation.
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31446136
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2
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Sodium chloride
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Added to water samples to facilitate PEG-6000 precipitation process.
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3
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RNase-free water
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Used to resuspend the pellet obtained after centrifugation.
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4
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Geneaid Viral Nucleic acid Extraction Kit II (Geneaid, Taiwan)
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Used for extracting viral genomic DNA from concentrated water samples.
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5
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Primers specific for the NS1 gene of BuV
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Used in nested-PCR for BuV screening by amplifying the NS1 region.
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6
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Gel/PCR DNA Fragment Extraction kit (Geneaid, Taiwan)
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Used to purify PCR amplicons after agarose gel electrophoresis.
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7
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BigDye Terminator V3.1 Cycle Sequencing Reaction Kit (Applied Biosystems, USA)
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Used for sequencing the purified PCR product by a fluorescence-based cycle sequencing method.
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8
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Primers targeting the VP2 capsid region (BuV1VP2-F: 5′- ATGACTGAC ACACAAGATGTATCTGA - 3′ and ARUB261: 5′-TTASWWTGTGTAGTTWGGCATDSMTC - 3′)
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Used for amplification of the VP2 region of the detected BuV.
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