Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV013266 | GenBank Accession | MT024126 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 510 |
| Definition | Human bocavirus 1 isolate CMH-N049-13 capsid protein VP1 gene, partial cds. | Submission Date | 2020-02-05 |
| Strain Name | CMH-N049-13 | Sampling Date | from 2012-01 to 2018-12 |
| Sampling Country | Thailand | Location | Chiang Mai city, Thailand, Thailand |
| Submitted By | Nantachit,N. | Submitting Institution | Department of Microbiology, Chiang Mai University, Intavaroros Road, Chiang Mai 50200, Thailand |
| Original Host | Homo sapiens | Sample Type | fecal specimens |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Phosphate-buffered saline | To prepare 10% suspensions of fecal specimens. | 33486373 | View Related Publication |
| 2 | Geneaid Viral Nucleic acid Extraction Kit II (Geneaid, Taiwan) | To extract viral genome from the fecal specimens' suspensions. | ||
| 3 | GoTaq DNA polymerase (Promega, USA) | To perform nested-PCR assays for detecting human bocavirus. | ||
| 4 | RedSafe (South Korea) | To stain PCR products for visualization under UV-transilluminator. | ||
| 5 | Gel/PCR DNA Fragment Extraction kit (Geneaid, Taiwan) | To purify positive amplicons for nucleotide sequencing. | ||
| 6 | BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, USA) | For direct sequencing of PCR products. |