ParvoDB ID PV012977 GenBank Accession JF272508
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 2106
Definition Human bocavirus isolate TJ155-2009 VP1 protein and VP2 protein genes, complete cds. Submission Date 2011-01-30
Strain Name TJ155-2009 Sampling Date 2009-02-05
Sampling Country China Location Tianjin, China
Submitted By Li,X., Chen,J., Kong,M., Su,X., Zou,M., Guo,L. and Zhang,H. Submitting Institution Institute of Pathogenic Biology, Tianjin Centers for Disease Control and Prevention, No. 76 Hualong Road, Hedong District, Tianjin 300011, China
Original Host Homo sapiens Sample Type Nasopharyngeal aspirates sample
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Nuclisenseasy MAG 自动核酸提取仪 用于病毒核酸提取 https://www.zgggws.com/en/article/doi/10.11847/zgggws2011-27-11-14 View Related Publication
2 标本液用量 (200 μL) 提供标本液用于核酸提取
3 50 μL 洗脱液 用于核酸洗脱
4 PCR 检测HBoV NP-1基因片段
5 引物 (用于HBoV检测) PCR检测NP-1基因片段
6 4对引物 扩增HBoV VP1 /VP2基因的不同片段
7 ABI 373测序仪 (美国Applied Biosystems公司) 用于HBoV序列测定
8 SeeplexTMRV Detection kit-1 多重RT-PCR检测其他常见呼吸道病毒
9 Revert AidTM First Strand cDNA Kit cDNA第一链合成