Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV012675 GenBank Accession JX680591
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 455
Definition Human parvovirus B19 isolate 3195-2010 minor capsid protein VP1 gene, partial cds. Submission Date 2012-09-12
Strain Name 3195-2010 Sampling Date 2010-05-10
Sampling Country France Location France
Submitted By Leveque,N. Submitting Institution EA-4684/SFR CAP-SANTE, Laboratoire de Virologie Medical et Moleculaire, Faculte de Medecine, Universite de Reims Champagne-Ardenne, CHU de Reims, Avenue du General Koenig, Reims 51100, France
Original Host Homo sapiens Sample Type plasma samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 NucliSens easyMAG instrument (bioMerieux, Lyon, France) Total nucleic acids extraction from 200 ml of plasma specimens. 23588740 View Related Publication
2 TaqMan B19V quantitative real-time PCR assay (DICO Extra-r gene, Argene bioMerieux, Verniolle, France) Quantitation of Parvovirus B19 DNA.
3 iCycler IQ (Bio-Rad, Marnes-la-Coquette, France) Execution of the real-time PCR assay.
4 Oligonucleotide primers and fluorogenic probe (positions specified) Amplification and detection of the B19V NS1 protein gene.
5 iQ supermix 2X (Bio-Rad, Marnes-la-Coquette, France) Reagent mixture containing dNTPs, MgCl2, and iTaq DNA polymerase for PCR.
6 WHO International Standard for Parvovirus B19 DNA NAT assays 98/802 (NIBSC, Potters Bar, United Kingdom) Reference curve for quantitation of B19V DNA.
7 EBV R-gene kit (Argene bioMerieux) Detection and quantification of Epstein-Barr virus.
8 Commercial or 'in-house' quantitative real-time PCR assays Detection and quantification of CMV, EBV, HHV-6, BKV, and adenovirus from nucleic acids extract.
9 Nested-PCR targeted the NS1-VP1u junction Amplification of B19V DNA in positive plasma samples for sequencing.
10 ABI PRISM BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems, Carlsbad, CA) Sequencing of PCR products.
11 Enzyme ImmunoAssay kits (DiaSorin, Chivasso, Italie) Testing for Parvovirus B19 specific IgG and IgM.