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1
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Serum from a 68-year-old male
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Source of B19V inoculum
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27074255
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View Related Publication
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2
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Stool samples from a Brazilian child
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Source of HAV inoculum (strain HAF-203)
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3
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Bioelisa HAV 96T Kit
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Detection of total anti-HAV antibodies
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4
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Biotrin International Ltd assay
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Detection of IgG anti-B19V antibodies
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5
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Horiba ABX
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Determination of haematological parameters
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6
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May-Grünwald-Giemsa stain
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Staining of samples for microscopic examination
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7
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Abbott (US)
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Measurement of alanine aminotransferase (ALT) levels
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8
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QIAquick DNA Mini Kit; Qiagen (UK)
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Purification of polymerase chain reaction (PCR) products
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9
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Big Dye Terminator Cycle Sequencing Kit
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Direct sequencing of purified DNA fragments
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10
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Applied Biosystems 3130 Genetic Analyzer
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Sequence analysis of DNA fragments
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11
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Mouse monoclonal antibodies
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Primary antibodies for immunofluorescence detection of B19V and HAV antigens
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12
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Alexa Fluor 488-labelled chicken anti-mouse IgG
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Secondary antibody for immunofluorescence detection
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13
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Evans Blue and Slow Fade with DAPI
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Counterstaining slides
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14
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SYBR Green PCR Master mix; Qiagen
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Quantification of B19V DNA using real-time PCR
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15
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TaqMan system (Applied Biosystems 7500 Real-Time PCR System)
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Quantification of HAV RNA using RT-PCR
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