Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV012657 | GenBank Accession | MF784509 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 343 |
| Definition | Human bocavirus isolate 187 NP-1 gene, partial cds. | Submission Date | 2017-08-29 |
| Strain Name | 187 | Sampling Date | 2010-11 |
| Sampling Country | Gabon | Location | in Libreville (the capital of Gabon), in Franceville (southeast), in Oyem (north) and in Koulamoutou (south), Gabon |
| Submitted By | Lekana-Douki,S.E., Behillil,S., Enouf,V., Leroy,E.M. and Berthet,N. | Submitting Institution | Zoonosis and Emerging viral Diseases, Centre International de Recherches Medicales de Franceville (CIRMF), CIRMF, Franceville, Haut-Ogooue BP769, Gabon |
| Original Host | Homo sapiens | Sample Type | nasal swabs and stool samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Nasal swabs '553C' (Copan, Diagnostic) | Collect nasal specimens for virological investigation. | 30029615 | View Related Publication |
| 2 | BioRobot EZ1 workstation (Qiagen) | Extract DNA from samples. | ||
| 3 | EZ1 Virus Mini Kit version 2.0 (Qiagen) | Used in combination with the BioRobot EZ1 workstation for DNA extraction. | ||
| 4 | Real-time polymerase chain reaction (PCR) with forward primer 5′-CTG GGG CTC ATA TCA TCA -3′, reverse primer 5′-TCT CCC TCG TCT TCA TCA -3′, and probe 5′-AAC ACC CAA TCA GCC ACC T-3′ | Detect all types of HBoV using a 93 bp virus nucleoprotein (NP) target. | ||
| 5 | Master Mix Fast (Applied Biosystems) | Prepare the reaction mixture for real-time PCR. | ||
| 6 | 7500 Fast Real Time PCR system | Thermocycler used to run the PCR reaction. | ||
| 7 | DNA Taq polymerase kit (Invitrogen) | Amplify the NP-1 gene fragment using conventional PCR method for genotyping bocaviruses. |