Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV012615 | GenBank Accession | AY211616 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 471 |
| Definition | B19 virus isolation-source patient 2 VP1/2 protein gene, partial cds. | Submission Date | 2002-12-20 |
| Strain Name | None | Sampling Date | from 2000-07 to 2001-10 |
| Sampling Country | Germany | Location | the northern part of Germany, Germany |
| Submitted By | Lehmann,H.W., Knoell,A., Kuester,R.-M. and Modrow,S. | Submitting Institution | Institute of Medical Microbiology, University of Regensburg, Franz-Josef-Strauss Allee 11, Regensburg 93053, Germany |
| Original Host | Homo sapiens | Sample Type | serum and synovial fluid samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Protein A beads | Isolation of IgG-complexed virus from serum and synovial fluid | 12794831 | View Related Publication |
| 2 | Sigma-Aldrich | Source of Protein A–Sepharose beads for virus immunocomplex isolation | ||
| 3 | QIAquick spin columns (Qiagen) | DNA purification from the supernatant and immunocomplex-bound beads | ||
| 4 | Triton X-100 | Buffer component for incubation with Protein A beads | ||
| 5 | Mikrogen RecomBlot assays | Detection of IgG and IgM antibodies against parvovirus B19 | ||
| 6 | Biotrin ELISA | Determination of IgM and IgG antibodies against VP2 capsids | ||
| 7 | Qiagen QIAmp blood kit | DNA purification of inhibited serum and synovial fluid samples | ||
| 8 | Perkin-Elmer/Applied Biosystems ABI PRISM 7700 | DNA amplification for detecting parvovirus B19 genomes | ||
| 9 | Plasmid pJB | Standard reference for B19 genome quantification in TaqMan PCR assay | ||
| 10 | Qiagen QIAquick spin columns | Purification of PCR fragments for DNA sequence analysis |