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1
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KB or HeLa cells
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Host cells for growing AAV-2H
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6246271
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View Related Publication
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2
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Adenovirus type 2
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Helper virus required for AAV-2H multiplication
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3
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CsCl
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Purification of virus by banding
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4
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Trypsin and deoxycholate
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Lysis of infected cells
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5
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[3H]thymidine
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Labeling DNA for purification
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6
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Restriction endonucleases (SmaI, BglI, HaeIII, PstI, HpaII)
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Digestion of DNA for sequence and analysis
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7
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Bacterial alkaline phosphatase
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Remove phosphate groups
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|
8
|
T4 polynucleotide kinase
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Labeling 5' ends of DNA with 32P
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|
9
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[32P]ATP
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Radiolabeled ATP for 5' end labeling of DNA
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|
10
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6% polyacrylamide gels
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Separation of DNA cleavage products
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11
|
20%, 15%, 12% polyacrylamide gels
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Separation of specific DNA fragments and strands
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12
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Tris borate-EDTA (TBE) buffer
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Buffer for running gels
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13
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100 mM and 25 mM magnesium acetate
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Included in stop solutions for sequencing
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|
14
|
1 M piperidine
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Reaction component for DNA sequencing
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|
15
|
Flat-back dial thermometer
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Measuring gel temperature during electrophoresis
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