Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV012559 | GenBank Accession | EF450724 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 5277 |
| Definition | Human bocavirus isolate HK8, complete genome. | Submission Date | 2007-02-22 |
| Strain Name | HK8 | Sampling Date | |
| Sampling Country | China | Location | Hong Kong, China |
| Submitted By | Lau,S.K.P., Yip,C.C.Y., Que,T.L., Lee,R.A., Au-yeung,R., Zhou,B., So,L.Y., Lau,Y.L., Chan,K.H., Woo,P.C.Y. and Yuen,K.Y. | Submitting Institution | Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam Road, Hong Kong, China |
| Original Host | Homo sapiens | Sample Type | fecal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp DNA Mini Kit (Qiagen) | Extract DNA from NPAs and fecal samples. | 17763318 | View Related Publication |
| 2 | Forward primer 5′-GAGCTCTGTAAGTACTATTAC-3′ | Polymerase chain reaction (PCR) for HBoV targeting a 354-bp fragment of the NP1 gene. | ||
| 3 | Reverse primer 5′-CTCTGTGTTGACTGAATACAG-3′ | Polymerase chain reaction (PCR) for HBoV targeting a 354-bp fragment of the NP1 gene. | ||
| 4 | ABI Prism 3700 DNA Analyzer (Applied Biosystems) | Sequence both strands of all PCR products. | ||
| 5 | QIAamp Viral RNA Mini Kit (Qiagen) | Extract viral RNA from NPAs. | ||
| 6 | SuperScript II Kit (Invitrogen) | Perform reverse transcription using random hexamers. |