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1
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0.01M PBS, pH 7.2
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Preparation of 30% fecal suspension
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27272684
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View Related Publication
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2
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QIAamp viral isolation mini kit (QIAGEN, Hilden, Germany)
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Extraction of total nucleic acid from fecal suspensions
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3
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DakoCytomation ELISA (Ely, Cambs, UK)
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Detection of group A rotavirus
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4
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Taq Polymerase (Roche, Germany)
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PCR amplification first round
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5
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10X PCR buffer
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PCR reaction component
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6
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dNTP (Promega, Madison, WI, USA), 10mM concentration
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PCR reaction component
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7
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PCR primers (targeting RdRp, Hexon, ORF1a, 5’UTR regions)
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Detection of NoV, AdV, AstV, and EV by PCR
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8
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Primers targeting VP1/VP2 region (~575bp)
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Nested PCR for HBoV detection
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9
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2% agarose gel with ethidium bromide (0.05%)
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Separation and visualization of PCR products
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10
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QIAquick gel purification kit (Qiagen, Germany)
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Purification of PCR amplicons
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11
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BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Austin, TX, USA)
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Sequencing purified PCR products
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