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1
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Polyethylene glycol (PEG) 6000
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Used to concentrate virus particles in environmental water samples by precipitation.
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34937184
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2
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NaCl
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Used in combination with PEG 6000 to aid in precipitation of virus particles from environmental water samples.
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3
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RNase-free water
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Used to resuspend the pellet of virus particles after centrifugation.
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4
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Geneaid Viral Nucleic Acid Extraction Kit
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Used to extract viral nucleic acid from the concentrated samples.
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5
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GoTaq DNA Polymerase (Promega, USA)
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Used in nested PCR for amplification of the VP1/VP2 region of the HBoV genome.
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6
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First-round primers AK-VP-F1 and AK-VP-R1
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Used in the first round of nested PCR to amplify the VP1/VP2 region of the HBoV genome.
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7
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Second-round primers AK-VP-F2 and AK-VP-R2
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Used in the second round of nested PCR to further amplify the VP1/VP2 region of the HBoV genome.
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8
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Gel/PCR DNA Fragment Extraction Kit (Geneaid, Taiwan)
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Used to purify the PCR products before sequencing.
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9
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NanoDrop Spectrophotometer
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Used to check the DNA quality and quantify the purified PCR product.
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10
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Sufficient and optimal concentration of purified PCR product (20 ng/mL) with AK-VP-F2 primer
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Used for direct nucleotide sequencing.
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11
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BLAST (Basic Local Alignment Search Tool)
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Used to compare nucleotide sequences of HBoV strains detected in this study with reference sequences in the NCBI GenBank database.
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12
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MEGA X software
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Used to construct a phylogenetic tree of partial VP1/VP2 regions.
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