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1
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NucliSens extractor (NucliSens, bioMerieux, Boxtel, The Netherlands)
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Isolation of nucleic acid from manufacturing pools, test pools, and individual donations.
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17845257
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2
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LightCycler instrument (Roche Diagnostics, Mannheim, Germany)
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Running real-time polymerase chain reaction (PCR) assays.
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3
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B19V DNA quantification assay from Roche
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Amplification of a fragment within the non-structural protein (NS1) gene for quantitative detection of B19V DNA.
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4
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Primers EVF and EVR
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Used in an in-house developed B19V DNA assay for reliable detection and quantification of B19V genotypes 1, 2, and 3.
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5
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Modified hydrolysis probe with LNA bases at specific sites (5' (FAM)- AAC.CCC.GCG.CTC.TAG.TAC-(BBQ3) 3')
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Used in the in-house assay to improve robustness and detection accuracy of B19V DNA.
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6
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ABI PRISM BigDye terminator cycle sequencing ready reaction kit (version 3.0) (Applied Biosystems/Hitachi, Nieuwerkerk a/d IJssel, The Netherlands)
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Sequencing of purified PCR products.
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7
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ABI PRISM 3130xl Genetic Analyzer
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Performing sequence analysis of PCR products.
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8
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SYBR green
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Staining of PCR products for agarose gel electrophoresis to analyze the intensity of amplified DNA bands.
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9
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Panel of primers (multiple forward and reverse primers listed in Table 1)
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Used for sequencing specific regions of B19V to identify genotypic differences and mismatches.
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